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abcb5  (Novus Biologicals)


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    Structured Review

    Novus Biologicals abcb5
    Abcb5, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+abcb5/ABCB5+Antibody+%5BAlexa+Fluor%C2%AE+488%5D/pm41720850-178-55-57
    Average 94 stars, based on 1 article reviews
    abcb5 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Gentle:

    Article Title: Dual-procedural separation of CTCs in cutaneous melanoma provides useful information for both molecular diagnosis and prognosis
    Article Snippet: The cells were cultured in RPMI-1640 medium (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific) and 1% penicillin-streptomycin-glutamine (Thermo Fisher Scientific). .. After gentle cell detachment from culture flasks, the expression of membrane antigens was explored by incubating 1 × 10 5 cells with 5 μl fluorescein isothiocyanate (FITC)-conjugated anti-CD271 (Bio-Legend, CA), anti-ABCB5 (Novus Biologicals, MN), and anti-RANK (Novus Biological) MoAbs, as well with phycoerythrin (PE)-conjugated anti-MCAM/CD146 (Becton-Dickinson), anti-N-cadherin (N-Cad; Invitrogen, CA), and anti-CD44 (Bio-Legend) MoAbs. .. After 30 min of incubation at 4°C, cells were analyzed for their antigenic profile by BD AccuriTM C6-plus (Becton Dickinson, CA).

    Expressing:

    Article Title: Dual-procedural separation of CTCs in cutaneous melanoma provides useful information for both molecular diagnosis and prognosis
    Article Snippet: The cells were cultured in RPMI-1640 medium (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific) and 1% penicillin-streptomycin-glutamine (Thermo Fisher Scientific). .. After gentle cell detachment from culture flasks, the expression of membrane antigens was explored by incubating 1 × 10 5 cells with 5 μl fluorescein isothiocyanate (FITC)-conjugated anti-CD271 (Bio-Legend, CA), anti-ABCB5 (Novus Biologicals, MN), and anti-RANK (Novus Biological) MoAbs, as well with phycoerythrin (PE)-conjugated anti-MCAM/CD146 (Becton-Dickinson), anti-N-cadherin (N-Cad; Invitrogen, CA), and anti-CD44 (Bio-Legend) MoAbs. .. After 30 min of incubation at 4°C, cells were analyzed for their antigenic profile by BD AccuriTM C6-plus (Becton Dickinson, CA).

    Membrane:

    Article Title: Dual-procedural separation of CTCs in cutaneous melanoma provides useful information for both molecular diagnosis and prognosis
    Article Snippet: The cells were cultured in RPMI-1640 medium (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific) and 1% penicillin-streptomycin-glutamine (Thermo Fisher Scientific). .. After gentle cell detachment from culture flasks, the expression of membrane antigens was explored by incubating 1 × 10 5 cells with 5 μl fluorescein isothiocyanate (FITC)-conjugated anti-CD271 (Bio-Legend, CA), anti-ABCB5 (Novus Biologicals, MN), and anti-RANK (Novus Biological) MoAbs, as well with phycoerythrin (PE)-conjugated anti-MCAM/CD146 (Becton-Dickinson), anti-N-cadherin (N-Cad; Invitrogen, CA), and anti-CD44 (Bio-Legend) MoAbs. .. After 30 min of incubation at 4°C, cells were analyzed for their antigenic profile by BD AccuriTM C6-plus (Becton Dickinson, CA).

    Staining:

    Article Title: Circulating tumor cells from melanoma patients show phenotypic plasticity and metastatic potential in xenograft NOD.CB17 mice
    Article Snippet: Briefly, ten ml of peripheral blood were collected in the CellSave tube and processed using Ficoll gradient (Histopaque®-1077-Sigma-Aldrich) while CD45 + cells and Glycophorin + cells were depleted by AutoMACS® cell separator (Miltenyi Biotec). .. The negative fraction was stained with a cocktail of three different monoclonal antibodies (MoAbs) toward putative cancer stem cell (CSC) antigens as anti-CD271 (Bio-Legend), anti-RANK (Novus Biological) and anti-ABCB5 (Novus Biologicals) MoAbs labelled with fluorescein isothiocyanate (FITC), as well as mesenchymal (M) antigens including anti-MCAM/CD146 (Becton-Dickinson), anti-N-cadherin (N-Cad; Invitrogen) and anti-CD44 (Bio-Legend) Moabs labelled with phycoerythrin (PE). .. Both mononuclear cells and endothelial cells were excluded from the cocktail of allophycocyanin (APC) conjugated MoAbs (anti-CD45, −CD31 and -CD34; Invitrogen).

    Bioprocessing:

    Article Title: Circulating tumor cells from melanoma patients show phenotypic plasticity and metastatic potential in xenograft NOD.CB17 mice
    Article Snippet: Briefly, ten ml of peripheral blood were collected in the CellSave tube and processed using Ficoll gradient (Histopaque®-1077-Sigma-Aldrich) while CD45 + cells and Glycophorin + cells were depleted by AutoMACS® cell separator (Miltenyi Biotec). .. The negative fraction was stained with a cocktail of three different monoclonal antibodies (MoAbs) toward putative cancer stem cell (CSC) antigens as anti-CD271 (Bio-Legend), anti-RANK (Novus Biological) and anti-ABCB5 (Novus Biologicals) MoAbs labelled with fluorescein isothiocyanate (FITC), as well as mesenchymal (M) antigens including anti-MCAM/CD146 (Becton-Dickinson), anti-N-cadherin (N-Cad; Invitrogen) and anti-CD44 (Bio-Legend) Moabs labelled with phycoerythrin (PE). .. Both mononuclear cells and endothelial cells were excluded from the cocktail of allophycocyanin (APC) conjugated MoAbs (anti-CD45, −CD31 and -CD34; Invitrogen).



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    Immunohistochemical findings of limbal stem cell markers in the limbal epithelium of chronic graft-versus-host disease (cGVHD) mice. Sections of the limbus from syngeneic control mice and cGVHD mice treated with vehicle, stem cells from human exfoliated deciduous teeth-conditioned medium (SHED-CM), immortalized-SHED-CM (IM-SHED-CM), or >3.5 kD fractionated components were stained for ATP-binding cassette sub-family B member 5 <t>(ABCB5)</t> (green) (a) or p63 (green) (b) (n = 5–6, per group). The corneal epithelium is located above the white dotted line. Data are presented as the mean ± standard error of the mean. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. One-way analysis of variance with Tukey–Kramer's post-hoc test was used for the analysis. Scale bar, 10 μm.
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    Image Search Results


    Immunohistochemical findings of limbal stem cell markers in the limbal epithelium of chronic graft-versus-host disease (cGVHD) mice. Sections of the limbus from syngeneic control mice and cGVHD mice treated with vehicle, stem cells from human exfoliated deciduous teeth-conditioned medium (SHED-CM), immortalized-SHED-CM (IM-SHED-CM), or >3.5 kD fractionated components were stained for ATP-binding cassette sub-family B member 5 (ABCB5) (green) (a) or p63 (green) (b) (n = 5–6, per group). The corneal epithelium is located above the white dotted line. Data are presented as the mean ± standard error of the mean. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. One-way analysis of variance with Tukey–Kramer's post-hoc test was used for the analysis. Scale bar, 10 μm.

    Journal: Regenerative Therapy

    Article Title: Conditioned media of stem cells from human exfoliated deciduous teeth contain factors related to extracellular matrix organization and promotes corneal epithelial wound healing

    doi: 10.1016/j.reth.2025.03.002

    Figure Lengend Snippet: Immunohistochemical findings of limbal stem cell markers in the limbal epithelium of chronic graft-versus-host disease (cGVHD) mice. Sections of the limbus from syngeneic control mice and cGVHD mice treated with vehicle, stem cells from human exfoliated deciduous teeth-conditioned medium (SHED-CM), immortalized-SHED-CM (IM-SHED-CM), or >3.5 kD fractionated components were stained for ATP-binding cassette sub-family B member 5 (ABCB5) (green) (a) or p63 (green) (b) (n = 5–6, per group). The corneal epithelium is located above the white dotted line. Data are presented as the mean ± standard error of the mean. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. One-way analysis of variance with Tukey–Kramer's post-hoc test was used for the analysis. Scale bar, 10 μm.

    Article Snippet: This process was applied to antibodies against p63 (dilution of 1:500, sc-25268, monoclonal, Santa Cruz Biotechnology, Dallas, TX), ATP-binding cassette sub-family B member 5 (ABCB5) (dilution of 1:500, bs-1604R, Bioss Antibodies, Woburn, MA), 4-hydrogy-2-nonenal (4-HNE) (dilution of 1:100, HNEJ-2, Japan Institute for the Control Aging, Shizuoka, Japan), or 8-hydroxy-2′-deoxyguanosine (8-OHdG) (dilution of 1:50, MOG-020P, N45.1, Genox, Torrance, CA).

    Techniques: Immunohistochemical staining, Control, Staining, Binding Assay

    Description of primary antibodies, their respective manufacturers, and dilutions

    Journal: Revista Brasileira de Ortopedia

    Article Title: Osteopontin, WNT3A, and ABCB5 Biomarkers Expression in Osteosarcoma Patients

    doi: 10.1055/s-0044-1788671

    Figure Lengend Snippet: Description of primary antibodies, their respective manufacturers, and dilutions

    Article Snippet: ABCB5 , Anti-ABCB5, clone 5H3C6 , GeneTex , 1:200.

    Techniques:

    Descrição dos anticorpos primários com seus respectivos fabricantes e diluições

    Journal: Revista Brasileira de Ortopedia

    Article Title: Osteopontin, WNT3A, and ABCB5 Biomarkers Expression in Osteosarcoma Patients

    doi: 10.1055/s-0044-1788671

    Figure Lengend Snippet: Descrição dos anticorpos primários com seus respectivos fabricantes e diluições

    Article Snippet: ABCB5 , Anti-ABCB5, clone 5H3C6 , GeneTex , 1:200.

    Techniques:

    Figure 1. Topology of ABCB5FL and ABCB5β. Two-dimensional representation of the topology of ABCB5FL and ABCB5β based on CCTOP prediction

    Journal: Cancer drug resistance (Alhambra, Calif.)

    Article Title: The uniqueness of ABCB5 as a full transporter ABCB5FL and a half-transporter-like ABCB5β.

    doi: 10.20517/cdr.2024.56

    Figure Lengend Snippet: Figure 1. Topology of ABCB5FL and ABCB5β. Two-dimensional representation of the topology of ABCB5FL and ABCB5β based on CCTOP prediction

    Article Snippet: In melanoma or retinoblastoma cell culture, ABCB5 protein (600-401-A77 antibody from Rockland) or mRNA expression did not correlate with CD271 or CD133 expression[48,56].

    Techniques:

    Figure 2. Tissue and intracellular localization of ABCB5FL and ABCB5β. (A) Representation of human ABCB5 tissue localization based on ABCB5FL and ABCB5β mRNA detection from

    Journal: Cancer drug resistance (Alhambra, Calif.)

    Article Title: The uniqueness of ABCB5 as a full transporter ABCB5FL and a half-transporter-like ABCB5β.

    doi: 10.20517/cdr.2024.56

    Figure Lengend Snippet: Figure 2. Tissue and intracellular localization of ABCB5FL and ABCB5β. (A) Representation of human ABCB5 tissue localization based on ABCB5FL and ABCB5β mRNA detection from

    Article Snippet: In melanoma or retinoblastoma cell culture, ABCB5 protein (600-401-A77 antibody from Rockland) or mRNA expression did not correlate with CD271 or CD133 expression[48,56].

    Techniques: